t98g cell lines  (ATCC)

Journal: The Journal of Biological Chemistry

Article Title: The role of DNAJC3 in enhancing glioma progression and regulating the tumor immune microenvironment

doi: 10.1016/j.jbc.2025.111059

Figure Lengend Snippet: The ERS-related protein DNAJC3 is significantly overexpressed in glioma. A , volcano map showing the differentially expressed proteins (|log 2 FC| ≥1, and p < 0.01). B , Venn diagram analysis of differentially expressed proteins and ERS-related proteins (relevance score >2). C , expression levels of DNAJC3 in glioma tissues (n = 3, Student's t test, compared with adjacent). D , changes in the expression levels of GRP78 and DNAJC3 in T98G and U251 cells after treatment with different concentrations of tunicamycin (n = 3, ANOVA, compared with 0 μM). The data are presented as mean ± SD. (∗∗∗∗ p < 0.0001; ∗∗∗ p < 0.001; ∗∗ p < 0.01; ∗ p < 0.05). DNAJC3, DnaJ heat shock protein family member C3; ERS, endoplasmic reticulum stress; FC, fold change.

Article Snippet: The human GBM cell lines T98G and U251 were purchased from Procell Life Science & Technology Co, Ltd.

Techniques: Expressing

Journal: The Journal of Biological Chemistry

Article Title: The role of DNAJC3 in enhancing glioma progression and regulating the tumor immune microenvironment

doi: 10.1016/j.jbc.2025.111059

Figure Lengend Snippet: In vitro , overexpression of DNAJC3 promotes the proliferation, migration, invasion, and antiapoptosis ability of T98G and U251 cells. A , effects of high/low expression of DNAJC3 on cell viability were assessed using the CCK-8 assay (n = 3, Student's t test, compared with si-NC/vector). B , the impact of high/low expression of DNAJC3 on cell proliferation capacity was examined through colony formation assay (n = 3, Student's t test, compared with si-NC/vector). C , the influence of high/low expression of DNAJC3 on cell migration ability was evaluated using a wound healing assay (n = 3, ANOVA, compared with si-NC/vector). D , the effect of high/low expression of DNAJC3 on cell migration was assessed using a transwell assay (magnification: 40×) (n = 3, ANOVA, compared with si-NC/vector). E , the impact of high/low expression of DNAJC3 on cell invasion ability was determined using a transwell invasion assay (n = 3, ANOVA, compared with si-NC/vector). F , the effect of high/low expression of DNAJC3 on cell apoptosis was analyzed using flow cytometry (n = 3, Student's t test, compared with si-NC/vector). The data are presented as mean ± SD. (∗∗∗∗ p < 0.0001; ∗∗∗ p < 0.001; ∗∗ p < 0.01; ∗ p < 0.05; CON, control group; DNAJC3, DNAJC3 overexpression group; si-DNAJC3, DNAJC3 knockdown group; si-NC, negative control of the knockdown group; vector, empty vector control of the overexpression group.) CCK-8, Cell Counting Kit-8; DNAJC3, DnaJ heat shock protein family member C3.

Article Snippet: The human GBM cell lines T98G and U251 were purchased from Procell Life Science & Technology Co, Ltd.

Techniques: In Vitro, Over Expression, Migration, Expressing, CCK-8 Assay, Plasmid Preparation, Colony Assay, Wound Healing Assay, Transwell Assay, Transwell Invasion Assay, Flow Cytometry, Control, Knockdown, Negative Control, Cell Counting